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Protocols, guides and teaching material
Methods are only useful if they travel. These are the protocols and teaching resources I am comfortable sharing — use, adapt and improve them.
Laboratory protocols
β-propeller phytase purification SOP
BugBuster lysis → HisPur Ni-NTA → SDS-PAGE / Western → dual-pH activity assay. No phosphate, no EDTA, calcium held throughout.
Dual-sgRNA design & Golden Gate assembly
Designing multiplex guide cassettes, screening for internal restriction sites, and assembling into single-transcript vectors.
PEG-mediated protoplast transformation
Isolation, transfection and recovery for transient and DNA-free editing.
PCR & colony-screening guide
Primer design conventions, cycling parameters and a troubleshooting table for stubborn amplicons.
Teaching & slides
Intro to CRISPR/Cas for plant scientists
Lecture slides: the biology, the toolbox, and how outcomes are classified.
Biosafety of gene-edited crops (Malaysia)
Seminar deck on the Biosafety Act 2007, NBB, GMAC and where SDN editing fits.
Reading a phylogenetic tree
A short teaching module on model selection, support values and common misreadings.
Reproducible lab-notebook template
A structured template that makes methods easy to reuse and audit.
Links are placeholders. Upload each file to a /files folder in public_html and point the href at it — no database needed.