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Portfolio
A working portfolio across genome editing, plant regeneration and enzyme production. Each is an ongoing line of work rather than a finished story.
Targeted edits in local rice backgrounds, from guide design through regeneration and outcome analysis, using single-transcript-unit vectors.
PEG-mediated delivery of plasmids and ribonucleoproteins to isolated protoplasts, enabling transient and DNA-free editing workflows.
Reproducible regeneration of edited cells into whole plants — the step that most often decides whether an editing project succeeds.
Recombinant β-propeller phytase produced in E. coli and Priestia, captured on Ni-NTA and assayed across pH, with no phosphate buffers or EDTA.
A multi-layered in silico and experimental strategy to keep the enzyme active through feed-pelleting heat, focused on calcium coordination and loop rigidity.
Model-based phylogenetics and homology modelling that feed directly back into isolate selection and mutation choices.
How a project runs
Numbered here because the order genuinely matters — each step gates the next.
Choose targets, design guides, screen for internal restriction sites, and model outcomes in silico.
Golden Gate assembly of guide cassettes, with sequence verification before anything touches a cell.
Transform protoplasts or explants, then regenerate edited cells into whole plants.
Sequence edits, decompose outcomes, classify them, and evaluate the trait.